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Project C.2.7

Axial slices of 3D tomography of human kidney tissue samples ex vivo. Normal kidney tissue (A/D/G), angiomyolipoma (AML) (B/E/H) and clear cell carcinoma (ccRCC) (C/F/I) visualized by absorption, dark field imaging and PCI. PCI images potentially allow the discrimination between normal tissue with vessels and even discrete differentiation between cortex and medulla (G), AML presented with homogenous tissue (H) and ccRCC with separate compact tumor cell clusters (I) (Image: Institut für Klinische Radiologie, Ludwig-Maximilians-Universität München)


The aim of this project is to evaluate and establish grating-based phase contrast imaging (PCI) for visualization and characterization of renal parenchymal diseases and renal masses. We will employ the following steps: 

(1) use and optimize the technical grating-based PCI at the TUM Garching to acquire 3D tomographies of phase contrast, absorption contrast and dark-field signals of kidney parenchyma ex vivo;

(2) compare PCI, dark-field and absorption image characteristics for visualizing and differentiation of  parenchymal renal disease ex vivo using animal models;

(3) investigate PCI characteristics of explanted renal transplants in correlation to CT or MRI images and histopathology;

(4) compare PCI image characteristics of normal human kidney tissue and renal masses ex vivo with unenhanced CT, MRI (see also Fig. C.2.7) and histopathology;

Various animal models for parenchymal (e.g. acute renal transplant rejection) or glomerular diseases (e.g. glomerulonephritis, diabetes mellitus) will be employed.