Multispectral imaging allows the separation of various tissues and contrast agents in static or dynamic X-ray investigations with strongly enhanced accuracy and efficiency. However, this method has limitations with respect to overlapping spectra and radiation dose. Thus, we will systematically explore the feasibility of multispectral X-ray imaging using brilliant X-ray sources for an improved tissue characterisation at low doses (objective b): (1) We will initially simulate the physical processes using high-resolution voxel models and perform phantom studies to characterise possibilities and limitations with 1 to 5 keV at the LUX source, ~30 keV at the up-graded LUX and BRIX source; ~50 to 90 keV at ESRF and, once available, at SPECTRE.
Also, in collaboration with C.2e, K-edges at higher photon energies will be identified. (2) We will utilise energies in the range of 1 to 5 keV for proof-of-principle applications including initial in vivo experiments in embryonic zebrafish (and a mouse model using 20 to 40 keV). Measurements will be performed at ESRF and with LUX and BRIX in CALA. In addition to iodine (to visualise lung perfusion), we will investigate xenon gas (for lung ventilation), uric acid (as substrate of gout) and calcium, magnesium and sulfur (as constituents of renal calculi).