To evaluate the feasibility and establish grating-based PCI for characterisation of diffuse lung diseases in an in vivo animal model (objective c), we will closely collaborate with O. Eickelberg (Comprehensive Pneumology Center and Helmholtz Center Munich) and (1) optimise the technical grating-based setup at the TUM Garching using excised animal lung tissue (see Fig. C.2.3); (2) compare PCI image characteristics of lung tissue in in vivo animal models between cases and controls using the grating-interferometer experimental set-up and BRIX; (3) compare resulting PCI image characteristics to PCI images obtained at large synchrotron facilities (ESRF and DESY) and to established imaging techniques, i.e. CT, and histology.
Various mouse models for diffuse lung diseases (i.e. COPD, fibroproliferative diffuse lung disease, asthma) will be employed. Diseased and healthy lung tissue will be investigated. Phase contrast, absorption contrast and dark-field signals will be acquired as plain 2D projections and 3D tomographies. Analysed parameters will include: pathologic changes in the pulmonary interstitium, small airways and pulmonary (micro-) vasculature.